on column dnase digestion (Qiagen)
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On Column Dnase Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 2019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/column+dnase+digestion/RNase-Free+DNase+Set/pmc13141651-199-25-28
Average 98 stars, based on 2019 article reviews
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other:Article Title: MECP2 Duplication Uncouples Mitochondrial and Purine Metabolism During neuronal maturation Article Snippet: RNA from cell cultures was harvested using either the Article Title: Long-read genome and RNA sequencing resolve a pathogenic intronic germline LINE-1 insertion in APC Article Snippet: Cell pellets of lymphoblastoid cell lines were stored in RNAprotect solution before RNA was extracted using the Article Title: MECP2 Duplication Uncouples Mitochondrial and Purine Metabolism During neuronal maturation Article Snippet: RNA from cell cultures was harvested using either the Centrifugation:Article Title: Telomere Dysfunction and Proteostasis Decline Define Distinct Pathways of Cellular Senescence in the Human Respiratory Tract. Article Snippet: .. Bound RNA was washed with 350 μL RW1 buffer and centrifuged for 30 s at 8000 × g. On- Isolation:Article Title: Fasting boosts breast cancer therapy efficacy via glucocorticoid activation Article Snippet: MCF7 xenograft tumour RNA was isolated by homogenizing the tissue sample in 1 ml of RLT buffer (79216, Qiagen) and 1% β-mercaptoethanol using the Qiagen TissueLyserII (85300, Qiagen) for 6 min with frequency setting 30 (1 s −1 ) in combination with 5-mm stainless steel beads (69989, Qiagen). .. The total RNA was isolated using the Article Title: Fasting boosts breast cancer therapy efficacy via glucocorticoid activation. Article Snippet: MCF7 xenograft tumour RNA was isolated by homogenizing the tissue sample in 1 ml of RLT buffer (79216, Qiagen) and 1% β-mercaptoethanol using the Qiagen TissueLyserII (85300, Qiagen) for 6 min with frequency setting 30 (1 s−1) in combination with 5-mm stainless steel beads (69989, Qiagen). .. The total RNA was isolated using the Article Title: Neutrophils Display Novel Partners of Cytosolic Proliferating Cell Nuclear Antigen Involved in Interferon Response in COVID-19 Patients. Article Snippet: After the desired incubation period (see the Results section), ultrapure HD neutrophils were pelleted by centrifugation and then total RNA was extracted by RNeasy mini kit (Qiagen). .. To completely remove any possible contaminating DNA, an on-column DNase digestion with the Purification:Article Title: Suppression of hepatic amino acid catabolism by the human-specific lncRNA hLMR1 Article Snippet: .. RNA was then extracted using Trizol–chloroform, and the aqueous phase was purified using RNeasy Mini columns following the manufacturer’s clean-up and |
